Thermo Fisher Scientific Ku80 Monoclonal Antibody (111)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.4 - 1 µg/mL

View 29 publications 29 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:200

View 11 publications 11 publications

Flow Cytometry (Flow)

0.5 µg/test

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mL

View 7 publications 7 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Gel Shift (GS)

-

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Published species

Fruit fly, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

111

Immunogen

Human B cell nuclei from plasmacytoid 2p68 cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ku80,uniProtId:P13010-1,ncbiNodeId:9606,antigenRange:1-732,antigenLength:732,antigenImageFileName:MA5-12933_Ku80_P13010-1_House_mouse.svg,antigenImageFileNamePDP:MA5-12933_Ku80_P13010-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10983840

Product Specific Information

MA5-12933 targets Ku (p80) in FACS, ICC/IF, IP, and WB applications and shows reactivity with Human, Mouse and Non-human primate samples. This antibody does not cross-react with mouse in immunofluorescent applications, but does react with mouse in FACS applications.

The MA5-12933 immunogen is human B cell nuclei from plasmacytoid 2p68 cells.

Target Information

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.