Thermo Fisher Scientific MAPK12 Monoclonal Antibody (OTI10E1), TrueMAB
인간 MAPK12(p38 gamma)에 특이적인 Mouse IgG1 단일클론 항체. Western blot, IHC, Flow cytometry에 사용 가능. 무보존제, 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 세포 신호전달 및 스트레스 반응 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10E1 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human MAPK12 expression vector (target family: p38 MAPK gamma, UniProt ID: P53778-1, antigen range: 1–367) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAPK12 (p38 gamma) is a serine/threonine kinase involved in various cellular processes. It is activated during hypoxia and gamma-irradiation-induced G(2) arrest. Activation of mitogen-activated protein kinases is a key mechanism for extracellular signal transduction. MAPK12 acts as a signal transducer during myoblast differentiation into myotubes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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