Thermo Fisher Scientific Gal-Screen β-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells
세포 직접 용해와 고감도 화학발광 검출을 결합한 β-Galactosidase 리포터 유전자 분석 시스템. 포유류 및 효모 세포 모두 사용 가능하며, 고처리량 자동화에 적합. 비방사성 방식으로 1시간 내 빠른 결과 제공.
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Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells
The Gal-Screen™ assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.
주요 특징
- Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media or additional cell lysis step.
- Glow-assay kinetics provide a time window for measurement, facilitating HTS applications and assay automation.
- Compatible with both mammalian and yeast model systems for flexible experimental design.
- Wide dynamic range enables accurate enzyme measurement from femtogram to nanogram levels.
- Sensitivity is 100–1,000× higher than conventional CAT or colorimetric/fluorescent β-gal assays.
- High sensitivity with broad dynamic range permits detection of varying reporter levels without multiple dilutions.
- Non-radioactive assay eliminates radioisotope concerns.
- Complete assay in about one hour for fast turnaround.
Ideal for Screening
This homogeneous assay is ideally suited for screening applications requiring automation. The Gal-Screen™ system uses Galacton-Star™ chemiluminescent substrate for convenient measurement in a luminometer.
Light emission reaches maximum in 60–90 minutes and remains constant for 45–90 minutes.
For Research Use Only. Not for use in diagnostic procedures.
사양 (Specifications)
| 항목 | 내용 |
|---|---|
| 제품 유형 | β-Galactosidase Reporter Gene Assay System |
| 검출 방법 | Chemiluminescence |
| 사용 장비 | Luminometer (Microplate) |
| 어세이 | β-Gal (lacZ) Assay |
| 호환 셀 | Mammalian Cells, Yeast Cells |
| 고처리량 호환성 | High-throughput Compatible |
| 라벨 또는 염료 | Unconjugated |
| 판독 방식 | End Point |
| 샘플 종류 | Cell Cultures |
| 기질 | Galacton-Star™ |
| 기질 유형 | Beta-Gal Substrate |
| 기질 특성 | Chemical Substrate |
| 타겟 효소 | Beta-Galactosidase |
| 제품 라인 | Gal-Screen™, NovaBright™ |
| 용도 (애플리케이션) | Enzyme Assay |
| 수량 | 1000 Assays |
| 형태 | Liquid |
| Unit Size | 1,000 assays |
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