Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), Biotin, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), Biotin, eBioscience™

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 661
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available upon request

Product Specific Information

The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells), and at low levels on monocytes. CD4 acts as a receptor for human immunodeficiency virus (HIV).
The OKT4 antibody recognizes a distinct epitope from the RPA-T4 monoclonal antibody; these antibodies do not cross-block each other.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells at ≤0.125 µg per test (100 µL final volume).
Cell number can range from 10⁵–10⁸ cells/test. Antibody should be titrated for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD4 antigen participates in MHC class II molecule recognition and serves as a co-receptor for HIV. It is primarily expressed on T helper cells but can also be found on monocytes, macrophages, and dendritic cells.
Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of brain and gut.
CD4 contributes to early T-cell activation via interaction with T-cell receptor complex and Lck kinase, and may mediate neuronal damage in infectious or immune-mediated CNS diseases.
Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.