Thermo Fisher Scientific MLH1 Monoclonal Antibody (OTI1B9), TrueMAB
MLH1 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot 등에 적합. 인간 유래 재조합 단백질(aa 299-527)을 면역원으로 사용. 동결건조 형태이며 PBS/trehalose 버퍼에 보관. DNA 불일치 수복 연구 및 MSI 관련 연구에 활용 가능.
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Applications
- Western Blot (WB)
Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 299–527 of human MLH1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
MLH1 is a DNA mismatch repair protein essential for maintaining genetic integrity.
Defects in mismatch repair pathways can lead to microsatellite instability (MSI), which is associated with human carcinogenesis and hereditary nonpolyposis colon cancer (HNPCC).
MLH1 interacts with MSH2 and PMSH to facilitate the later steps of mismatch repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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