Thermo Fisher Scientific PHD3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A, with a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site.

Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated, allowing HIFs to escape degradation, translocate to the nucleus, heterodimerize with HIF1B, and increase expression of hypoxia-inducible genes.

EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Acts as a regulator of cardiomyocyte and neuronal apoptosis—disrupting the BAX-BCL2 complex in cardiomyocytes and regulating CASP3 activity in neurons. Essential for hypoxic regulation of neutrophilic inflammation and plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferentially recognized via a LXXLAP motif.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.