Thermo Fisher Scientific JMJD3 Polyclonal Antibody
JMJD3 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, ICC/IF, Flow cytometry, ChIP 등 다양한 응용 가능. 인간 및 마우스 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태, 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific JMJD3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | Assay-Dependent | - |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide made to an internal portion of the mouse protein (within residues 600–650). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151839 |
Product Specific Information
The target sequence has 100% sequence homology with rat.
Target Information
Covalent modification of histones plays a critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones.
A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate.
Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both demethylate histone 3 at lysine 9).
Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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