Thermo Fisher Scientific Phospho-NFkB p100 (Ser866) Polyclonal Antibody
NFkB p100 (Ser866) 인산화 부위를 인식하는 토끼 폴리클로날 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 친화성 크로마토그래피 정제, 액상 형태, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 866 (E-D-S(p)-A-Y) derived from Human NF-kappa-B-p100/p52 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554272 |
Product Specific Information
- Suggested positive control for Western blot: ovary cancer cells
- Suggested positive control for IHC: human breast carcinoma
- Suggested positive control for ICC/IF: HeLa cells
Target Information
This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can act as a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, potentially forming fusion proteins. A pseudogene for this gene exists on chromosome 18. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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