Thermo Fisher Scientific ADAR1 Polyclonal Antibody
ADAR1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. 인간 시료 반응성이 높으며, 항원 친화 크로마토그래피로 정제된 액상 형태입니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific ADAR1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 472–757 of ADAR1 (Uniprot ID: P55265) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11154508 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2
- Predicted reactivity: Mouse (80%), Rat (80%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Adenosine Deaminase RNA Specific (ADAR) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA), known as A-to-I RNA editing. This process can affect gene expression and function in various ways, including mRNA translation, pre-mRNA splicing, RNA stability, and viral genome replication. ADAR edits both viral and cellular RNAs, either at multiple sites (hyper-editing) or specific sites (site-specific editing).
Cellular RNA substrates include:
- BLCAP (bladder cancer-associated protein)
- GRIA2 (glutamate receptor)
- HTR2C (serotonin receptor)
- GABRA3 (GABA receptor)
Viral RNA substrates include:
- HCV, VSV, MV, HDV, HIV-1
ADAR1 exhibits both proviral and antiviral effects depending on the virus and mechanism (editing-dependent or independent). High levels of ADAR1 can inhibit HDV replication.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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