Servicebio Electrophoresis System (Transfer)

Servicebio Electrophoresis System (Transfer)

Product Introduction

This system is designed for use with SVE-2 vertical electrophoresis. It transfers proteins, nucleic acids, and other charged molecules from the gel to a transfer membrane using an electric field for subsequent biochemical analysis.

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Product Features

• Double plate structure compatible with SVE-2 electrophoresis tank
• High transparency polycarbonate tank, corrosion-resistant and durable
• Distinct positive and negative electrodes with handle for easy operation
• High transfer efficiency, typical transfer time 20–40 minutes
• Strong structure allows synchronized ice bath cooling to prevent overheating and ensure reliable results

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Product Specifications

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Operation Process

4.1 Preparation

Prepare 8 pieces of 7×9 cm thin transfer membrane filter paper or 2 pieces of thick filter paper of the same specification, and 1 piece of 7×9 cm PVDF membrane.
Use 0.45 μm pore size for proteins >20 kDa, and 0.2 μm for proteins <20 kDa.

4.2 Activation

Activate the PVDF membrane with methanol for 2 minutes before use.

4.3 Installation

Follow the sequence:
positive electrode (+) → sponge → filter paper → transfer membrane → gel → filter paper → sponge → negative electrode (–)
Remove air bubbles during assembly. The red side represents the positive direction, and the black side the negative direction. Place the transfer clip into the transfer core, aligning red to red and black to black. The transfer core accommodates two clips simultaneously.

Black: Negative
Red: Positive
Sponge / filter paper / transfer membrane / gel / filter paper / sponge

4.4 Power-On Transfer

Connect the electrophoresis power supply, set transfer conditions, and start the membrane transfer.

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Product Configuration List

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Precautions

1. Handle carefully; main components are fragile and may be damaged by impact or dropping.
2. Inspect platinum wire and electrode columns regularly for oxidation, breakage, or loosening; replace or maintain as needed.
3. After each use, rinse with deionized water and dry in a dust-free area.

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Related Product Classification

• Protein Biology Reagents | Consumables
• Western Blotting
• Transfer Consumables Instruments

Servicebio is a leading manufacturer in China providing FDA & CE approved laboratory supplies and services.

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Reference
Yu, J., et al. Quercetin Ameliorates Lipopolysaccharide-Induced Duodenal Inflammation through Modulating Autophagy, Programmed Cell Death and Intestinal Mucosal Barrier Function in Chicken Embryos. Animals (Basel) 12, 3524 (2022). PMID: 36552443, IF 3

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제품 이미지

Electrophoresis tank cover / Transfer clip / Trans-Blot module / Electrophoresis tank