Thermo Fisher Scientific Torc1 Monoclonal Antibody (1B5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1B5

Immunogen

Purified recombinant fragment of human CRTC1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10980185

Product Specific Information

MA5-15666 targets CRTC1 in indirect ELISA, FACS, IF, and WB applications and shows reactivity with Human samples.

The MA5-15666 immunogen is purified recombinant fragment of human CRTC1 expressed in E. Coli.

.

MA5-15666 detects CRTC1 which has a predicted molecular weight of approximately 67kDa.

Target Information

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 Ser-133 phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons. In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.