Thermo Fisher Scientific IL-12 p35 Polyclonal Antibody
IL-12 p35 폴리클로날 항체로 인간, 마우스, 랫트 반응성. Western blot 및 IHC(P) 적용 가능. 항원 친화 크로마토그래피로 정제된 라이오필라이즈드 형태. IL-12A 관련 면역 신호 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived rat IL12A recombinant protein (Position: R23–S215) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2746576 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Interleukin 12A (IL-12A) is a signaling cytokine belonging to the Interleukin-6 protein superfamily. It encodes a 35-kDa subunit of the disulfide-linked heterodimer cytokine IL-12, exhibiting diverse biological activities.
IL-12A acts as a growth factor for activated T and NK cells, enhances NK/lymphokine-activated killer cell lytic activity, and stimulates IFN-γ production by resting PBMCs.
It contributes to differentiation of Th1 and Th2 cells and positively regulates tyrosine phosphorylation of Stat4 protein.
IL-12A signaling in innate immunity requires nitric oxide synthase 2A. Together with EBI3, IL-12A forms interleukin-35 (IL-35), a heterodimeric cytokine produced by Treg cells with suppressive activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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