Thermo Fisher Scientific Phospho-p27 Kip1 (Ser10) Polyclonal Antibody
인산화된 p27 Kip1(Ser10)을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P)에서 사용 가능. 인간에 반응하며, 단백질 정제는 Protein A 및 항원 친화 크로마토그래피로 수행. 세포주기 조절 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human p27 Kip1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10988518 |
Product Specific Information
This antibody is predicted to react with hamster and mouse based on sequence homology.
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and may act as a mediator of TGFβ-induced G1 arrest. It is a key regulator of cell cycle progression and a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms complexes with cyclin D-CDK4, influencing their assembly, stability, and activation. Its phosphorylation state determines whether it acts as an inhibitor or activator. The protein (~27 kDa) undergoes CDK-dependent phosphorylation and ubiquitination by SCF complexes, leading to its degradation and enabling the transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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