Thermo Fisher Scientific CD68 Monoclonal Antibody (C68, 684)
CD68 단일클론 항체(C68, 684)는 인간, 마우스, 비인간 영장류, 랫트에 반응하며 다양한 응용(WB, IHC, ICC, Flow)에 적합합니다. 단백질 A/G로 정제된 액상 형태로 제공되며, 면역조직화학 및 세포 분석에 유용합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C68, 684 |
| Immunogen | Recombinant human CD68 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on intracellular lysosomes of monocytes and macrophages, and to a lesser extent by dendritic cells and peripheral blood granulocytes.
CD68 may play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell or cell-pathogen interactions.
It is expressed by interdigitating reticulum cells in tonsil and in some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma.
Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several AML studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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