Thermo Fisher Scientific CD68 Recombinant Mouse Monoclonal Antibody (rLAMP4, 824)
CD68 단백질을 인식하는 Mouse Recombinant Monoclonal Antibody로, 인간 시료에 반응합니다. Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능하며, 고순도 Protein A/G 정제 및 안정적 PBS buffer 제공. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rLAMP4, 824 |
| Immunogen | Recombinant full-length human LAMP4 (CD68) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on intracellular lysosomes of monocytes and macrophages, and to a lesser extent by dendritic cells and peripheral blood granulocytes.
It may play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
CD68 is expressed by interdigitating reticulum cells in tonsil and certain histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma.
Elevated CD68 expression has been demonstrated on CD34+ cells in various human malignancies, including several AML studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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