Thermo Fisher Scientific Phospho-Estrogen Receptor alpha (Tyr537) Polyclonal Antibody
Phospho-Estrogen Receptor alpha (Tyr537) 폴리클로날 항체로 인간, 생쥐, 랫트 시료에 반응. Western blot, IHC, ICC, Flow 등 다양한 응용 가능. KLH 결합 합성 인산화 펩타이드로 면역화. Protein A 정제, 액상 형태, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human ER alpha around the phosphorylation site of Tyr537 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen hormone, induces a conformational change allowing dimerization and binding to estrogen response elements (ERE) in DNA. ER can regulate gene transcription positively or negatively through recruitment of coactivator or corepressor proteins.
There are two forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2). At least four estrogen receptor-alpha isoforms are known to exist due to alternative RNA splicing (Isoform 1: 66 kDa, Isoform 2: 53 kDa, Isoform 3: 47 kDa, Isoform 4: 35 kDa). Estrogen receptors are widely expressed and are essential for sexual development, reproductive function, and bone health. They are implicated in diseases such as breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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