Thermo Fisher Scientific NDFIP2 Polyclonal Antibody
NDFIP2 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot과 IHC(파라핀)에서 검증됨. 사람 및 생쥐 시료에 반응하며, 친화 크로마토그래피로 정제된 1 mg/mL 액상 제품. 연구용으로 세포 내 NDFIP2 발현 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NDFIP2 (Accession Q9NV92), corresponding to amino acid residues G61–E111. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2850378 |
Product Specific Information
Antibody detects endogenous levels of total NDFIP2.
Target Information
NDFIP2 activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1, and WWP2, thereby modulating the stability of their targets and controlling various cellular processes. It recruits ITCH, NEDD4, and SMURF2 to endosomal membranes and negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and promoting degradation through NEDD4L-mediated ubiquitination. NDFIP2 may also modulate EGFR signaling and, together with NDFIP1, limits cytokine signaling and effector Th2 T-cell expansion by promoting degradation of JAK1 via ITCH- and NEDD4L-mediated ubiquitination. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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