Thermo Fisher Scientific Diphtheria Toxin Monoclonal Antibody (E53J)

Applications

Tested Dilution

Publications

ELISA (ELISA)

1:20 - 1:200

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

E53J

Immunogen

C. diphtheria lysate.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_558642

Product Specific Information

MA1-7027 detects the Diphtheria toxin. This antibody is specific for the intact toxin/toxoid, and is non-reactive with free A or B subunits.

MA1-7027 has been successfully used in ELISA procedures.

The MA1-7027 immunogen is C. diphtheria lysate.

Target Information

Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.