Thermo Fisher Scientific NUSAP1 Polyclonal Antibody

Applications and Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:500–1:1,000

Immunocytochemistry (ICC/IF)

• Tested Concentration: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
KLLKALKGYI KHEARKGNEN QDESQTSASS CDETEIQISN QEEAERQPLG HVTKTRRRCK TVRVDPDSQQ NHSEIKISNP TEFQNHEKQE SQDFRATAKV PSPPDEHQEA ENAVSSGNRD SKV

• Highest antigen sequence identity to the following orthologs:
  • Mouse: 50%
  • Rat: 49%

Target Information

Ubiquitin:
Exists either covalently attached to another protein or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains), or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains).

Polyubiquitin chains have different functions depending on the Lys residue linkage:

• Lys-6: DNA repair
• Lys-11: ERAD and cell-cycle regulation
• Lys-29: Lysosomal degradation
• Lys-33: Kinase modification
• Lys-48: Protein degradation via proteasome
• Lys-63: Endocytosis, DNA-damage response, NF-kappa-B activation

Linear polymer chains formed via attachment by the initiator Met lead to cell signaling.
Conjugation usually occurs at Lys residues, but rarely at Cys or Ser residues.
Free polyubiquitin plays distinct roles such as activation of protein kinases and signaling.

60S ribosomal protein L40:
Component of the 60S subunit of the ribosome. Essential for translation of a subset of cellular transcripts, especially for cap-dependent translation of vesicular stomatitis virus mRNAs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.