Thermo Fisher Scientific HLA-DP/-DR (MHC II) Monoclonal Antibody (Bra-14)
인간 HLA-DP/-DR(MHC II) 인식용 mouse monoclonal antibody (Clone Bra-14). IHC, ICC/IF, Flow Cytometry 등 다양한 면역실험에 사용 가능. Unconjugated, 200 µg/mL 농도, Protein A/G 정제. 면역계 항원 제시 연구에 적합.
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Thermo Fisher Scientific HLA-DP/-DR (MHC II) Monoclonal Antibody (Bra-14)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG3, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Bra-14 |
| Immunogen | Human REH cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen-presenting cells (APC: B lymphocytes, dendritic cells, macrophages).
The alpha chain is approximately 33–35 kDa and its gene contains 5 exons:
- Exon 1 encodes the leader peptide
- Exons 2 and 3 encode the two extracellular domains
- Exon 4 encodes the transmembrane domain and the cytoplasmic tail
DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5.
HLA and MHC antibodies play a significant role in immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 3115-MSM2-P0_HLA-DRDP_P01911-1_House_mouse.svg, 3115-MSM2-P0_HLA-DRDP_P04440-1_House_mouse.svg 등)
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