Thermo Fisher Scientific NFKBIB Monoclonal Antibody (1B5)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

ELISA (ELISA)

1 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1B5

Immunogen

NFKBIB (AAH15528, 56 a.a. approximately 145 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IkB beta,uniProtId:Q15653-1,ncbiNodeId:9606,antigenRange:56-145,antigenLength:356,antigenImageFileName:H00004793-M01_IkB_beta_Q15653-1_House_mouse.svg,antigenImageFileNamePDP:H00004793-M01_IkB_beta_Q15653-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: EDGDTALHLA VIHQHEPFLD FLLGFSAGTE YMDLQNDLGQ TALHLAAILG ETSTVEKLYA AGAGLCVAER RGHTALHLAC RVGAHACARA

Target Information

The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB. The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKK alpha and IKK beta serve as the catalytic subunits of the kinase and IKK gamma serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKK beta (Ser176 and Ser180 in IKK alpha), which causes conformational changes, resulting in kinase activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.