Thermo Fisher Scientific NFKBIB Monoclonal Antibody (1B5)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 5 µg/mL

ELISA

• Tested Dilution: 1 ng/mL

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
EDGDTALHLA VIHQHEPFLD FLLGFSAGTE YMDLQNDLGQ TALHLAAILG ETSTVEKLYA AGAGLCVAER RGHTALHLAC RVGAHACARA

Target Information

The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB.
The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits: IKK alpha and IKK beta (catalytic subunits) and IKK gamma (regulatory subunit).
Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKK beta (Ser176 and Ser180 in IKK alpha), which causes conformational changes resulting in kinase activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.