Thermo Fisher Scientific Human GPR15L Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

KRRPAKAWSG RRTRLCCHRV PSPNSTNLKG HHVRLCKPCK LEPEPRLWVV PGALPQV

Molecular weight

6.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human PBMC`s in a transwell-based migration assay.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

PeproTech`s E.coli-derived Recombinant Human GPR15L consists of 57 amino acid residues and has a calculated molecular weight of 6.5 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

GPR15L is a newly identified ligand for GPR15, a member of the G protein-coupled receptor (GPCR) family. Upon ligation, GPR15L acts as a potent chemoattractant for GPR15-expressing T cells and together they mediate lymphocyte recruitment to the large intestine and skin. GPR15L is constitutively expressed by colon epithelial cells where its expression is minimally altered by intestinal inflammation. Conversely, GPR15L is nearly undetectable in adult epidermis but highly upregulated during wound healing and inflammation, particularly in psoriasis. Significant expression of GPR15L is also seen in additional mucosal epithelial cells, including those of the stomach, esophagus, and urinary tract. While maintaining similar expression patterns and intramolecular disulfide cysteine bridges found in members of the CC chemokine family, GPR15L differs from classic CC and CXC chemokines, whose active sites are found on the N-terminus, in that it relies on its C-terminus for receptor interaction. GPR15L was first identified as an antimicrobial peptide (AMP) due to its broad spectrum of antimicrobial activity, a property shared with many chemokines. GPR15L binds to an additional receptor, SUSD2, and early studies have indicated that both are downregulated in colon cancer tissue resulting in inhibited colon cancer growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.