Thermo Fisher Scientific Mouse IL-6 (ELISA Standard) Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species

Mouse

Published species

Not Applicable

Expression System

E. coli

Amino acid sequence

Mouse IL-6, amino acids Phe25-Thr211 (Accession # MMINTL6)

Molecular weight

21-28 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Description: mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a ~21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported: Mouse IL-6 Lyophilized Standard has been reported for use in the Mouse IL-6 ELISA Ready-SET-Go! Set (Product # 88-7064).

Applications Tested: The intended use of the Mouse IL-6 Lyophilized Standard is as a standard for Mouse IL-6 ELISA (Product # 88-7064-88). Typically the standard is run in a series of 7 doubling dilutions from the top standard. Please refer to the Certificate of Analysis included with each ELISA set for instructions on the proper reconstitution and dilution of the lyophilized standard.

Note: These are single-use vials. Use one time within 4 hours of reconstitution and discard. Reconstitute and dilute as suggested in the Certificate of Analysis or recovery may not be accurate.

Source: E. coli expressed amino acids Phe 25-Thre 211 of mature mouse IL-6 accession # MMINTL6.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.