Thermo Fisher Scientific nNOS Polyclonal Antibody
nNOS 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ELISA 등 다양한 응용에 사용 가능. eNOS 및 iNOS와 교차반응 없음. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼에 0.25 mg/mL 농도로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 13 publications |
| Immunohistochemistry (IHC) | - | 50 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL | 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1–2 µg/mL | 1 publication |
| Immunohistochemistry – Free Floating (IHC (Free)) | - | 3 publications |
| Immunocytochemistry (ICC/IF) | - | 8 publications |
| ELISA | 0.1–1 µg/mL | - |
| Immunoprecipitation (IP) | - | 2 publications |
| Immunomicroscopy (IM) | - | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein consisting of 195 amino acids from the N-terminus of rat nNOS protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2313734 |
Product Specific Information
This antibody reacts with the ~160 kDa nNOS protein and does not exhibit cross-reactivity with eNOS or iNOS proteins. Reactivity was confirmed with a ~160 kDa band on western blots of rat and mouse brain tissue lysates (20 µg). In immunohistochemistry with paraffin-embedded tissues, some background staining was observed.
Target Information
NOS activity requires homodimerization and three cosubstrates (L-arginine, NADPH, and O₂), along with five cofactors (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Distinct NOS isoforms are products of three separate genes: NOS1 (ncNOS, neuronal), NOS2 (iNOS, inducible), and NOS3 (ecNOS, endothelial).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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