Thermo Fisher Scientific nNOS Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 13 publications 13 publications

Immunohistochemistry (IHC)

-

View 50 publications 50 publications

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

1-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-

View 8 publications 8 publications

ELISA (ELISA)

0.1-1 µg/mL

-

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Immunomicroscopy (IM)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A recombinant protein consisting of 195 amino acids from the N-terminus of rat nNOS protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:nNOS,uniProtId:P29476-1,ncbiNodeId:10114,antigenRange:1,antigenLength:1429,antigenImageFileName:61-7000_nNOS_P29476-1_Rabbit.svg,antigenImageFileNamePDP:61-7000_nNOS_P29476-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2313734

Product Specific Information

This antibody reacts with the ~160 kDa nNOS protein and does not exhibit any cross-reactivity with the related eNOS or iNOS proteins. During development reactivity was confirmed with a ~160 kD band on western blots of rat and mouse brain tissue lysates (20 µg). For use in immunohistochemistry with paraffin embedded tissues some background staining was evident.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.