Thermo Fisher Scientific nNOS Polyclonal Antibody
nNOS 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ELISA 등 다양한 응용에 사용 가능. eNOS 및 iNOS와 교차반응 없음. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼에 0.25 mg/mL 농도로 제공.
- 카탈로그번호
- 617000
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific nNOS Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 13 publications |
| Immunohistochemistry (IHC) | - | 50 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL | 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1–2 µg/mL | 1 publication |
| Immunohistochemistry – Free Floating (IHC (Free)) | - | 3 publications |
| Immunocytochemistry (ICC/IF) | - | 8 publications |
| ELISA | 0.1–1 µg/mL | - |
| Immunoprecipitation (IP) | - | 2 publications |
| Immunomicroscopy (IM) | - | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein consisting of 195 amino acids from the N-terminus of rat nNOS protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2313734 |
Product Specific Information
This antibody reacts with the ~160 kDa nNOS protein and does not exhibit cross-reactivity with eNOS or iNOS proteins. Reactivity was confirmed with a ~160 kDa band on western blots of rat and mouse brain tissue lysates (20 µg). In immunohistochemistry with paraffin-embedded tissues, some background staining was observed.
Target Information
NOS activity requires homodimerization and three cosubstrates (L-arginine, NADPH, and O₂), along with five cofactors (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Distinct NOS isoforms are products of three separate genes: NOS1 (ncNOS, neuronal), NOS2 (iNOS, inducible), and NOS3 (ecNOS, endothelial).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.