Thermo Fisher Scientific COX5A Polyclonal Antibody
COX5A 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, ELISA에 사용 가능. Human, Mouse, Rat에 반응하며, 미토콘드리아 내막 단백질 검출에 적합. 고농도(4.35 mg/mL) 액상 형태로 제공되며, -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:10–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–150 of human COX5A (NP_004246.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 4.35 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914575 |
Product Specific Information
Positive test controls include: HeLa, HepG2, mouse skeletal muscle, mouse testis, mouse heart, mouse brain, rat heart, rat kidney, Jurkat.
The target is usually localized in the mitochondrion inner membrane.
Immunogen sequence:
MLGAALRRCA VAATTRADPR GLLHSARTPG PAVAIQSVRC YSHGSQETDE EFDARWVTYF NKPDIDAWEL RKGINTLVTY DMVPEPKIID AALRACRRLN DFASTVRILE VVKDKAGPHK EIYPYVIQEL RPTLNELGIS TPEELGLDKV
Target Information
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain.
It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane.
The complex consists of 13 mitochondrial- and nuclear-encoded subunits.
Mitochondrially encoded subunits perform electron transfer and proton pumping activities, while nuclear-encoded subunits may be involved in regulation and assembly.
The COX5A gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme.
A pseudogene, COX5AP1, is located on chromosome 14q22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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