Thermo Fisher Scientific Phospho-PKC alpha (Thr497) Recombinant Rabbit Monoclonal Antibody (PKCaT497-F1), APC
인간, 마우스, 랫트 반응성을 가진 PKC alpha Thr497 인산화 형태를 인식하는 재조합 토끼 단일클론 항체입니다. APC 형광표지로 유세포분석에 적합하며, HEK293 발현 시스템에서 제조되었습니다. Protein A/G 정제, PBS-BSA 완충액 보존, 4°C 보관.
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Thermo Fisher Scientific Phospho-PKC alpha (Thr497) Recombinant Rabbit Monoclonal Antibody (PKCaT497-F1), APC
Applications
- Flow Cytometry (Flow): 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PKCaT497-F1 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr497 of human phospho PKC alpha |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage buffer | PBS with 0.20% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897299 |
Additional Formats
- Unconjugated (MA5-37116)
- FITC (MA5-37118)
- PE (MA5-37117)
- Request custom conjugation
Target Information
The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. PKC family members phosphorylate various protein targets and are involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters.
This kinase plays roles in cell adhesion, transformation, cell cycle checkpoint, and volume control. Knockout studies in mice suggest its fundamental role in cardiac contractility and Ca²⁺ handling in myocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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