Thermo Fisher Scientific BTLA Monoclonal Antibody (OTI4D6), TrueMAB
BTLA 단백질을 표적으로 하는 Mouse monoclonal antibody로 Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. Human 시료 반응성, Lyophilized 형태로 제공되며 -20°C에서 보관. 연구용으로 세포 신호 조절 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4D6 |
| Immunogen | Full length human recombinant protein of human BTLA produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
BTLA (B and T-lymphocyte attenuator) is a co-inhibitory receptor of the CD28 superfamily. It functions as a lymphocyte inhibitory receptor, regulating immune responses by inhibiting lymphocyte activation.
BTLA is constitutively expressed on most CD4+ and CD8+ T cells, with expression decreasing upon activation. It remains expressed on Th1 but not Th2 cells.
BTLA interacts with the tumor necrosis factor receptor superfamily member HVEM, forming a key pathway for lymphocyte activation and immune homeostasis regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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