Thermo Fisher Scientific ALKBH8 Polyclonal Antibody
인간 ALKBH8 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC(P)에서 1:50~1:200 희석 비율로 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보관. DNA 손상 후 생존 및 세포 생존 관련 연구에 적합.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ALKBH8 (Control fragment: RP-97368) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C (short term); -20°C (long term), avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2637872 |
Product Specific Information
- Immunogen sequence:
LPPGLMVVEE IISSEEEKML LESVDWTEDT DNQNSQKSLK HRRVKHFGYE FHYENNNVDK DKPLSGGLPD ICESFLEKWL RKGYIKHKPD QMTINQYEPG QG - Ortholog sequence identity:
- Mouse: 82%
- Rat: 84%
Target Information
ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. It performs the final step in the formation of 5-methylcarboxymethyl uridine, with preference for tRNA(Arg) and tRNA(Glu).
Additionally, through its dioxygenase domain, ALKBH8 catalyzes hydroxylation reactions forming 5-(S)-methoxycarbonylhydroxymethyluridine, preferentially in tRNA(Gly).
It is required for normal survival after DNA damage and may inhibit apoptosis, promoting cell survival and angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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