Thermo Fisher Scientific CUEDC2 Polyclonal Antibody
Human CUEDC2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP 등 다양한 응용에 사용 가능. 보존제가 없는 액상 형태로 고순도 정제. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| ELISA | 1:25,000–1:50,000 |
| Immunoprecipitation (IP) | 1–2 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human CUEDC2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787829 |
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human CUEDC2.
Target Information
The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, approximately 40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation.
CUE domains are present in eukaryotic proteins involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found.
CUEDC2 (CUE domain-containing protein 2) was identified through yeast two-hybrid screening as a protein interacting with the progesterone receptor (PR), promoting progesterone-induced PR degradation via the ubiquitin-proteasome pathway.
CUEDC2 also decreases PR sumoylation and interacts with IKK-alpha and IKK-beta, reducing NF-kappa-B activation by decreasing IKK activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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