Thermo Fisher Scientific Cytokeratin 8 (KRT8) Recombinant Rabbit Monoclonal Antibody (KRT8, 2174R)
Recombinant rabbit monoclonal antibody targeting Cytokeratin 8 (KRT8). Validated for WB and IHC(P) applications. High purity and specificity with Protein A/G purification. Suitable for use in various species including human and bovine. For rese...
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Human, Sheep, Pig, Rabbit |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | KRT8, 2174R |
| Immunogen | Recombinant full-length human KRT8 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12.
Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells.
Cytokeratin 8 typically dimerizes with keratin 18 to form intermediate filaments in simple single-layered epithelial cells.
This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation.
Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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