Thermo Fisher Scientific Phospho-S6 (Ser235) Polyclonal Antibody
Phospho-S6 (Ser235) 폴리클로날 항체로 Western blot 및 IHC(P) 분석에 적합. 사람과 생쥐에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS 기반 완충액에 보관하며, 단기 4°C, 장기 -20°C 보관 권장. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RPS6 (phospho Ser235). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.12 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736419 |
Product Specific Information
- Predicted Reactivity: Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Dog (100%), Chicken (100%), Bovine (100%)
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins.
This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit and belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases.
Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens, while dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through selective translation of particular classes of mRNA.
As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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