Thermo Fisher Scientific Nhe-1 Polyclonal Antibody
Nhe-1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 적합합니다. 인간, 마우스, 랫트 등 다양한 종에 반응하며, 액상 형태로 제공됩니다. 세포 내 pH 조절 및 세포골격 관련 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SLC9A1 (Accession P19634), corresponding to amino acid residues R516–E566 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2900551 |
Product Specific Information
This antibody detects endogenous levels of total SLC9A1.
Target Information
The Na⁺/H⁺ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by various signals such as growth factors, mitogens, neurotransmitters, and tumor promoters.
Nhe-1 also anchors actin filaments to maintain cortical cytoskeleton integrity via interactions with ERM proteins (ezrin, radixin, moesin).
A structural role independent of ion exchange activity has been proposed, and Nhe-1 is implicated in hypertension.
At least two isoforms of Nhe-1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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