Thermo Fisher Scientific HSP90 Monoclonal Antibody (4F3.E8), FITC
FITC 형광 표지된 HSP90 단클론 항체로 Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합함. 인간, 마우스, 식물, 랫트 반응성. 단백질 G 정제, 액상 형태, 4°C 암조건 보관. HSP90α/β 모두 검출 가능.
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Thermo Fisher Scientific HSP90 Monoclonal Antibody (4F3.E8), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Plant, Rat |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F3.E8 |
| Immunogen | Recombinant Human HSP90 purified from E. coli |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931638 |
Additional Formats
- APC (MA5-45183)
- PerCP (MA5-45185)
- Request custom conjugation
Product Specific Information
0.5 µg/mL of MA5-45184 was sufficient for detection of HSP90α in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 90 kDa. This antibody detects both alpha and beta forms of HSP90 equally well.
Target Information
HSP90 proteins are highly conserved molecular chaperones involved in signal transduction, protein folding, degradation, and morphologic evolution. They associate with cochaperones and assist in folding newly synthesized proteins or refolding denatured proteins after stress. Two major cytosolic forms exist: HSP90AA1 (inducible) and HSP90AB1 (constitutive), with mitochondrial variants also present.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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