Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (10C4), FITC, eBioscience
Mouse 및 Rat Bcl-2 단백질을 인식하는 FITC 결합 단클론 항체로, 세포 내 유세포 분석에 적합합니다. 항체는 10C4 클론으로 제작되었으며, Blue Laser(488 nm) 기반 형광 검출에 최적화되어 있습니다. 암세포 사멸 조절 연구용으로 활용됩니다.
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Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (10C4), FITC, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Intracellular)
- Tested Dilution: 5 µL (0.5 µg)/test
- Publications: View 4 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C4 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10734059 |
Additional Formats Available
Product Specific Information
The 10C4 monoclonal antibody reacts with mouse and rat Bcl-2, also known as B cell lymphoma 2.
Bcl-2 is a key member of the Bcl-2 family that regulates cell death and survival. It inhibits apoptosis by preventing cytochrome c release from mitochondria. Overexpression or mutation of Bcl-2 is associated with various cancers.
- Applications Reported: Intracellular flow cytometric analysis
- Applications Tested: Pre-titrated and tested on mouse splenocytes and thymocytes at 5 µL (0.5 µg)/test
- Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
- Immunogen: Murine Bcl-2 peptide (amino acids 61–76)
- Cross-reactivity: Rat Bcl-2
Target Information
BCL-2 is a regulator of apoptosis, capable of both inhibiting and promoting cell death through dimerization with other BCL-2 family members.
Overexpression of BCL-2 is linked to cancers such as B-cell lymphoma and prostate cancer.
Two transcript variants exist, differing in their C-terminal regions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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