Thermo Fisher Scientific BRCC3 Monoclonal Antibody (OTI6G7), TrueMAB
BRCC3 단백질을 인식하는 Mouse monoclonal antibody로 Western blot에 적합합니다. Human, Mouse, Rat 시료에 반응하며, carrier-free 형태로 제공되어 conjugation 실험에 활용 가능합니다. -20°C 보관, PBS/trohalose buffer.
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Applications
- Western Blot (WB)
Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6G7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 41–316 of human BRCC3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
BRCC36 forms a holoenzyme complex with BRCA1, BRCA2, RAD51, and BRCC45, exhibiting ubiquitin E3 ligase activity. Aberrant BRCC36 levels are observed in sporadic breast tumors, and depletion of BRCC36 or BRCC45 increases sensitivity to ionizing radiation and causes G2/M checkpoint defects. BRCC36 is essential for BRCA1 phosphorylation and nuclear foci formation following DNA damage, making it a potential target for treating radiation-resistant breast tumors. At least two isoforms of BRCC36 are known.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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