Thermo Fisher Scientific Influenza A PB1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

CUT&RUN (C&R)

-

- -

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to N-terminal of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1))

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2552264

Product Specific Information

Recommended positive controls: WSN virus infected DF1 10hr.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Influenza A virus contains eight single stranded, negative sense RNAs. Each RNA is associated with viral RNA-dependent RNA polymerase which consists of PA, PB1 and PB2 proteins. PB1 is the subunit involved in the catalytic activity of nucleotide polymerization and is involved in the initiation of transcription. Both PB1 and PB2 can be crosslinked to synthetic RNA with the 3` terminal sequence of viral RNA. PB1 and PB2 may also be involved in recognition of the promoter and/or replication origin on template viral RNA. PB2 is the cap 1-recognition protein, which binds to the cap structure of host mRNA. The cap structure then acts as a primer for transcription to produce viral mRNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.