Thermo Fisher Scientific Influenza A PB1 Polyclonal Antibody
Influenza A PB1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공됩니다. 바이러스 연구용으로 사용되며, 장기 보관 시 -20°C에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 4 publications |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| In vitro Assay (IV) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
| CUT&RUN (C&R) | - | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Virus |
| Published Species | Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to N-terminal of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1)) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552264 |
Product Specific Information
- Recommended positive controls: WSN virus infected DF1 (10 hr).
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Influenza A virus contains eight single-stranded, negative-sense RNAs. Each RNA is associated with viral RNA-dependent RNA polymerase, which consists of PA, PB1, and PB2 proteins. PB1 is the subunit involved in the catalytic activity of nucleotide polymerization and initiation of transcription. PB1 and PB2 can be crosslinked to synthetic RNA with the 3′ terminal sequence of viral RNA. PB1 and PB2 may also participate in recognition of the promoter or replication origin on template viral RNA. PB2 serves as the cap 1-recognition protein, binding to the cap structure of host mRNA, which then acts as a primer for transcription to produce viral mRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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