Thermo Fisher Scientific NR1I3 Monoclonal Antibody (OTI1B2), TrueMAB
인간 NR1I3 단백질을 표적으로 하는 단클론 항체로 Western blot 및 IHC(P) 실험에 적합. Mouse IgG2a 형식으로 제조되었으며, carrier-free 형태로 제공. 고순도 친화 크로마토그래피 정제, 안정적 PBS 버퍼에 보존.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–236 of human NR1I3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882)
Target Information
The NR1I3 gene encodes a member of the nuclear receptor superfamily and acts as a key regulator of xenobiotic and endobiotic metabolism.
This protein binds to DNA as a monomer or as a heterodimer with the retinoid X receptor, regulating transcription of genes involved in drug metabolism and bilirubin clearance (e.g., cytochrome P450 family).
Unlike most nuclear receptors, NR1I3 is constitutively active without ligand but can be modulated by agonists and inverse agonists.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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