Thermo Fisher Scientific PSD-95 Polyclonal Antibody
PSD-95 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. KLH-conjugated peptide 면역원 사용, 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1.0 µg/mL | 18 publications |
| Immunohistochemistry (IHC) | - | 43 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 5 publications |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200 | 14 publications |
| ELISA | 0.1–1.0 µg/mL | - |
| Immunoprecipitation (IP) | 5 µg | - |
| Miscellaneous PubMed (Misc) | - | 2 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Chicken, Human, Mouse, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from the C-terminal region of human PSD-95 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533914 |
Product Specific Information
This antibody recognizes the 95 kDa PSD-95 protein. Cross-reactivity with an unknown protein at ~50 kDa has been observed in western blots with rat brain homogenates, but not in mouse brain homogenates.
Positive reactivity was confirmed on western blots of rat brain homogenates and lysates of PSD-95-transfected HEK293 cells.
Target Information
Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is a membrane-associated protein found in postsynaptic density of forebrain neurons and some presynaptic structures in the cerebellum.
PSD-95 contains three PDZ domains, an SH3 domain, and a Guanylate Kinase (GuK) domain. It plays a role in clustering NMDA receptors and Shaker-type potassium channels at synaptic membranes in CNS neurons.
PSD-95 interacts with proteins containing a C-terminal T/SXV motif and with neuronal nitric oxide synthase (nNOS) through PDZ-PDZ interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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