Thermo Fisher Scientific ITGB1 Monoclonal Antibody (JB1B)
Human CD29 (Integrin beta1) 인식하는 Mouse monoclonal antibody (Clone JB1B). 다양한 응용(WB, IHC, ICC, Flow, ELISA, IP)에 사용 가능. Rat과 반응하지 않으며, 세포 부착 및 면역세포 이동 연구에 적합. 액상 형태로 4°C 또는 -20°C 보관.
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Thermo Fisher Scientific ITGB1 Monoclonal Antibody (JB1B)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1 µg/1x10^6 cells |
| ELISA | 0.1–1 µg/mL |
| Immunoprecipitation (IP) | 1–5 µg |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JB1B |
| Immunogen | Purified Human beta1 integrin (Integrin beta 1, CD29, UniProt ID: P05556-1, antigen range: 1–798) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C or -20°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_928075 |
Product Specific Information
- This antibody does not react with rat.
- Mouse anti-Human CD29 antibody, clone JB1B recognizes human integrin beta1 (CD29), also known as Fibronectin receptor subunit beta, Glycoprotein IIa, or VLA-4 subunit beta.
Target Information
ITGB1 (Integrin Subunit Beta 1, CD29) is a 110 kDa cell surface glycoprotein expressed widely across various cell types including all leukocytes.
It forms non-covalent heterodimers with multiple alpha chains (α1–α6), determining the binding properties of beta1 (VLA) integrins.
ITGB1 functions as a cell adhesion molecule found on platelets and contributes to extracellular matrix interactions.
Decreased ITGB1 expression correlates with tumor multidrug resistance. It is upregulated during leukocyte migration and plays a key role in immune cell trafficking.
ITGB1 also regulates inflammation-related processes such as tissue damage in inflammatory bowel disease.
Fibronectin and type I collagen enhance cytokine secretion via ITGB1-dependent pathways.
Two major isoforms, beta1A and beta1D, differ by 13 amino acids and show tissue-specific distribution—beta1A in most tissues, beta1D in cardiac and skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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