Thermo Fisher Scientific ADAM17 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.3 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

3.75 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

synthetic peptide LQRQNRVDSKETEC (Human), C terminus. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ADAM17,uniProtId:P78536-1,ncbiNodeId:9606,antigenRange:824,antigenLength:824,antigenImageFileName:PA1-31074_ADAM17_P78536-1_Goat.svg,antigenImageFileNamePDP:PA1-31074_ADAM17_P78536-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1960914

Product Specific Information

Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX13535-PEP)..

Expected to cross-react with Chinese Hamster due to sequence homology.

Detects a band of approximately 125-130 kDa (predicted molecular weight: 95 or when glycosylated 130 kDa).

Target Information

ADAM17 (disintegrin and metalloproteinase domain-containing protein 17) cleaves the membrane bound precusor of TNF-alpha to its mature soluble form. ADAM 17 is responsbile for the proteolytical release of soluble JAM3 from endothelial cells surface and the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1, and the amyloid precursor protein. It acts as an activator of the Notch pathway by mediating cleavage of Notch and generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Mutations in the gene can result in inflammatory skin and bowel disease, neonatal 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.