Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio1316H), Biotin, eBioscience
Mouse IL-13을 인식하는 중화능을 가진 Rat IgG1 단일클론 항체로, Biotin으로 표지되어 ELISA, ELISPOT, 중화 실험 등에 적합합니다. 0.5 mg/mL 액상 형태이며, Affinity chromatography로 정제되었습니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 13-7135-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio1316H), Biotin, eBioscience
Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio1316H), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | - | View 3 publications |
| ELISA | 0.25–1 µg/mL | View 6 publications |
| Neutralization | - | View 3 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1316H |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_763555 |
Additional Formats
Product Specific Information
Description:
The eBio1316H antibody reacts with mouse IL-13 and functions as a neutralizing antibody. Mouse interleukin-13 (IL-13), originally designated p600, is an 11.6 kDa cytokine secreted by activated T helper cells, CD8+ T cells, and NK cells. It shares many biological activities with IL-4, requiring the IL-4Rα chain for signal transduction. IL-13 suppresses macrophage cytotoxic activity, upregulates IL-1RA expression, and inhibits inflammatory cytokine production. It induces CD23 expression on B cells and enhances class II MHC gene expression. Mouse IL-13 is active on both mouse and human cells.
Applications Reported:
Useful for neutralization, ELISA detection, and ELISPOT detection.
Applications Tested:
The biotinylated eBio1316H antibody has been validated as a detection antibody in sandwich ELISA and ELISPOT assays for mouse IL-13, used in combination with purified eBio13A (14-7133) as capture antibody and recombinant mouse IL-13 (14-8131) as standard.
Recommended ELISA detection concentration: 0.25–1 µg/mL.
A standard curve using recombinant IL-13 ranging from 1000 pg/mL to 8 pg/mL should be included in each ELISA plate.
For intracellular staining of mouse IL-13, fluorochrome-labeled eBio13A antibody is recommended.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It regulates inflammatory and immune responses by down-regulating macrophage activity, inhibiting pro-inflammatory cytokines and chemokines, and is implicated in allergen-induced asthma. IL-13 also controls B cell maturation, differentiation, and promotes IgE isotype switching. Structurally, IL-13 is similar to IL-3, IL-4, IL-5, and GM-CSF, and its gene is located on chromosome 5q within a cytokine gene cluster close to IL4.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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