Thermo Fisher Scientific SPAG5 Polyclonal Antibody
SPAG5 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot에 적합합니다. 인공 합성 펩타이드를 면역원으로 사용했으며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며, -20°C에서 보관합니다.
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Applications
- Western Blot (WB): Tested dilution 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide: V(532)NQESRRAETLV(543) (human MAP126 protein, amino acids 532–543) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2194784 |
Product Specific Information
- PA1-4269 detects the human MAP126 protein in human samples.
- Successfully used in Western blot applications.
- Immunizing peptide corresponds to amino acid residues 532–543 of human MAP126 protein.
Target Information
SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST, or Astrin, is a 1,193 amino acid protein predominantly expressed in testis and at lower levels in liver, placenta, pancreas, colon, and thymus.
It localizes to spindle poles during prophase and throughout the spindle during metaphase and anaphase, playing an essential role in proper spindle formation and mitotic spindle regulation.
SPAG5 is involved in sister chromatid cohesion and separation, contributing to chromosome integrity. It forms a homodimer with a long stalk and globular head, creating aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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