Thermo Fisher Scientific PLEKHM1 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• Suggested positive control: human lung tissue lysate
• PA5-20857 can be used with blocking peptide PEP-0971.

Target Information

PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, which includes a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain.
It was identified through segregation analysis as a cause of osteopetrosis in humans.
PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7.
Monocytes from a patient homozygous for a mutated form of PLEKHM1 differentiated into osteoclasts normally but failed to form ruffled borders and showed little evidence of bone resorption when cultured on dentine discs.
Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.