Thermo Fisher Scientific HMGB2 Recombinant Rabbit Monoclonal Antibody (ARC1460)
HMGB2 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC/IF, ELISA, IP, ChIP, CUT&RUN 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며 HEK293 세포에서 발현된 IgG 형 항체입니다. 고순도 친화 크로마토그래피 정제 및 안정적 액상 형태로 제공됩니다.
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Thermo Fisher Scientific HMGB2 Recombinant Rabbit Monoclonal Antibody (ARC1460)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
| ChIP assay (ChIP) | 5 µg antibody for 10–15 µg Chromatin |
| CUT&RUN (C&R) | 10⁵ cells / 1 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1460 |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human HMGB2 (UniProt P26583) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.14 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849671 |
Product Specific Information
Immunogen sequence:
MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK TMSAKEKSKF EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS
Target Information
HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. These chromatin-associated proteins are widely distributed in the nucleus of higher eukaryotic cells. In vitro studies show that HMGB2 efficiently bends DNA and forms DNA circles, facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. It is also implicated in DNA end-joining during double-strand break repair and V(D)J recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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