Thermo Fisher Scientific Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Monoclonal Antibody (SPM115)
다양한 종에 반응하는 Cytokeratin (Acidic Type I) 단클론 항체로, 면역조직화학, 웨스턴블롯, 유세포분석 등에 사용됩니다. 단백질 A/G 정제, 보존제 무첨가, 액상 형태로 제공되며, 암 연구 및 상피세포 마커 분석에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Human, Mouse, Non-human primate, Rabbit, Reptile, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM115 |
| Immunogen | Human epidermal keratin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cytokeratin pan belongs to a subfamily of intermediate filament proteins characterized by remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in molecular weight between 40–68 kDa and have an isoelectric pH between 4.9–7.8.
The various epithelia in the human body express cytokeratins characteristic of their tissue type and differentiation stage.
Cytokeratin subtype expression patterns are widely used in distinguishing different epithelial malignancies. These antibodies assist in tumor differential diagnosis using immunohistochemistry, and are valuable tools in cytopathology and flow cytometric assays.
The composition of cytokeratin pairs varies with epithelial cell type, differentiation stage, growth environment, and disease state. Keratins are widely used as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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