Thermo Fisher Scientific CD3e Monoclonal Antibody (UCHT1), Alexa Fluor 647
인간 CD3e 항원을 인식하는 Mouse IgG1 단클론 항체로, Alexa Fluor 647 형광 표지. Flow Cytometry에 적합하며, 650/671 nm의 여기/방출 특성. PBS 기반 버퍼에 보관하며 4°C, 암조건에서 안정적 유지.
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Applications
- Flow Cytometry (Flow): 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHT1 |
| Immunogen | Human thymocytes followed by Sezary T cells |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 0.04 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.0325% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2633286 |
Additional Conjugate Formats
- Alexa Fluor 405
- Alexa Fluor 700
- APC
- FITC
- Pacific Blue
- Pacific Orange
- PE
- Qdot 605
- Custom conjugation available
Target Information
The CD3 subunit complex is crucial for transducing antigen-recognition signals into T cell cytoplasm and regulating TCR surface expression. Activation of T cells through the TCR involves CD3 gamma, delta, epsilon, and zeta subunits—members of the immunoglobulin superfamily encoded on chromosome 11.
These subunits contain cytoplasmic tails with tyrosine-based motifs that mediate signal transduction and may influence TCR-induced growth arrest, survival, and proliferation.
CD3 antigen is expressed on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells. Reduced T lymphocyte percentages can occur in autoimmune diseases. Defects in CD3 genes are linked to CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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