Thermo Fisher Scientific Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), APC, eBioscience

Thermo Fisher Scientific Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), APC, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 14 publications

Product Specifications

Available Formats

• Brilliant UV 395
• Brilliant Violet 711
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

This SDRNR monoclonal antibody recognizes human and mouse AKT (Protein Kinase B, PKB) when phosphorylated on Ser473.
AKT is a serine/threonine kinase involved in metabolism, proliferation, apoptosis/survival, and migration.
Phosphorylation at Thr308 (by PDK1) and Ser473 is required for full activation.
AKT promotes cell survival by inhibiting apoptosis through phosphorylation of Bad, Foxo1, c-Raf, and caspase-9.
Deregulation of AKT is implicated in many cancers and negatively regulated by PTEN and LY294002.

Specificity Verification: ELISA, flow cytometry, and western blotting.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining of normal human peripheral blood cells.
Use at 5 µL (0.5 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.

Staining Protocols

Protocol A (Two-step): For cytoplasmic proteins; offers flexibility for surface and intracellular detection.
Protocol B (One-step): For nuclear proteins; suitable for transcription factors and phosphorylated proteins.
Protocol C (Two-step Fixation/Methanol): Best for phospho-specific signaling discrimination; limited surface staining.
Detailed protocols available in BestProtocols®: Staining Intracellular Antigens for Flow Cytometry.

Optical Properties

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase regulating cell survival and proliferation.
Activated by insulin, PI3K, IGF1, and other growth factors.
Promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of forkhead transcription factors and caspase-9.
A major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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