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Thermo Fisher Scientific Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), APC, eBioscience

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인간 및 마우스의 AKT1(Ser473) 인산화 형태를 특이적으로 인식하는 SDRNR 단클론 항체입니다. APC 형광체로 결합되어 유세포 분석(Flow Cytometry)에 최적화되어 있으며, 세포 내 인산화 단백질 연구에 적합합니다. 4°C 암소 보관, 동결 금지.

브랜드: Thermo Fisher Scientific
카테고리: Monoclonal Antibodies
판매단위: pk

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마지막 업데이트 2025. 08. 05. 오전 01:01

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Thermo Fisher Scientific 17-9715-42 Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), APC, eBioscience 100 tests pk판매 단위 pk ·

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590,500원VAT 포함 649,550원

Thermo Fisher Scientific 17-9715-41 Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), APC, eBioscience 25 tests pk판매 단위 pk ·

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254,200원VAT 포함 279,620원

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Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), APC, eBioscience

Thermo Fisher Scientific Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), APC, eBioscience™

Applications and Tested Dilution

Application: Flow Cytometry (Intracellular staining)
Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 14 publications

Product Specifications

항목	내용
Species Reactivity	Human, Mouse
Published Species	Human, Mouse
Host / Isotype	Mouse / IgG2a, kappa
Recommended Isotype Control	Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	SDRNR
Conjugate	APC
Excitation / Emission Max	651 / 660 nm
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage Buffer	PBS (pH 7.2) with 0.2% BSA
Contains	0.09% sodium azide
Storage Conditions	4°C, store in dark, DO NOT FREEZE
Shipping Conditions	Ambient (domestic); Wet ice (international)
RRID	AB_2573309

Available Formats

Brilliant UV 395
Brilliant Violet 711
eFluor 450
FITC
PE
PE-Cyanine7
Request custom conjugation

Product Specific Information

This SDRNR monoclonal antibody recognizes human and mouse AKT (Protein Kinase B, PKB) when phosphorylated on Ser473.
AKT is a serine/threonine kinase involved in metabolism, proliferation, apoptosis/survival, and migration.
Phosphorylation at Thr308 (by PDK1) and Ser473 is required for full activation.
AKT promotes cell survival by inhibiting apoptosis through phosphorylation of Bad, Foxo1, c-Raf, and caspase-9.
Deregulation of AKT is implicated in many cancers and negatively regulated by PTEN and LY294002.

Specificity Verification: ELISA, flow cytometry, and western blotting.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining of normal human peripheral blood cells.
Use at 5 µL (0.5 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.

Staining Protocols

Protocol A (Two-step): For cytoplasmic proteins; offers flexibility for surface and intracellular detection.
Protocol B (One-step): For nuclear proteins; suitable for transcription factors and phosphorylated proteins.
Protocol C (Two-step Fixation/Methanol): Best for phospho-specific signaling discrimination; limited surface staining.
Detailed protocols available in BestProtocols®: Staining Intracellular Antigens for Flow Cytometry.

Optical Properties

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase regulating cell survival and proliferation.
Activated by insulin, PI3K, IGF1, and other growth factors.
Promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of forkhead transcription factors and caspase-9.
A major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.