Thermo Fisher Scientific Hexanoyl-Lysine adduct Monoclonal Antibody (5E8)
Hexanoyl-Lysine adduct(HEL) 변형 펩타이드 및 단백질을 특이적으로 검출하는 Mouse Monoclonal Antibody(5E8). Western blot, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용 가능. Protein G 정제, PBS/glycerol buffer, -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| ELISA | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5E8 |
| Immunogen | Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735215 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-27555 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine.
Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Hexanoyl-lysine (HEL) is a novel lipid peroxidation biomarker derived from the oxidation of omega-6 unsaturated fatty acids.
HEL may be used as a biomarker in omega-6 PUFA (polyunsaturated fatty acid) oxidation in living organisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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