Thermo Fisher Scientific NEDD8 Polyclonal Antibody
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Applications
Tested Dilution
Publications
Western Blot (WB)
1:500-1:1,000
Immunocytochemistry (ICC/IF)
1:50-1:100
Flow Cytometry (Flow)
1:50-1:100
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit / IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human NEDD8 aa 1-81 if (typeof window.$mangular === undefined
|| !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{
targetFamily:
NEDD8,
uniProtId:
Q15843-1,
ncbiNodeId:
9606,
antigenRange:
1-81,
antigenLength:
81,
antigenImageFileName:
PA5-109237_NEDD8_Q15843-1_Rabbit.svg,
antigenImageFileNamePDP:
PA5-109237_NEDD8_Q15843-1_Rabbit_PDP.jpeg,
sortOrder:
1}\]
; $mangular.isB2BCMGT = false
; $mangular.isEpitopesModalImageMultiSizeEnabled = true
;
View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}
Conjugate
Unconjugated Unconjugated Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854648
Product Specific Information
Positive Control: PC-3M, HepG2, MCF-7.
Target Information
NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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