Thermo Fisher Scientific Cortactin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.01-0.03 µg/mL

-

Product Specifications

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids WKASAGHAVSIA of Human Cortactin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2088286

Product Specific Information

Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX21374-PEP)..

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cortactin (Cttn) is a ubiquitous actin-binding protein that was originally identified as a substrate for Src. It contributes to the organization of the actin cytoskeleton and cell shape. Cortactin also plays a role in the formation of lamellipodia and cell migration. It is accumulated in peripheral, actin-enriched structures of cells suggesting that cortactin facilitates actin network formation. Cortactin has four major domains of interest the N-terminal acidic (NTA) and tandem repeats domains and the C-terminal proline-rich and SH3 Domains. NTA associates with the Arp2/3 and WASP complex at F-actin branches. Cortactin is involved in promoting cell motility and invasion, including a critical role in invadopodia, actin rich-subcellular protrusions associated with degradation of the ECM by cancer cells. Cortactin is phosphorylated by src family kinases at Y421, Y466, and Y482 and S405 and S418 that are phosphorylated by Erk family kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.