Thermo Fisher Scientific Cortactin Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.01–0.03 µg/mL

Product Specifications

Product Specific Information

• Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX21374-PEP).
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cortactin (Cttn) is a ubiquitous actin-binding protein originally identified as a substrate for Src. It contributes to actin cytoskeleton organization and cell shape, and plays a role in lamellipodia formation and cell migration. Cortactin accumulates in actin-enriched peripheral structures, facilitating actin network formation.

It has four major domains: N-terminal acidic (NTA), tandem repeats, proline-rich, and SH3 domains. NTA associates with the Arp2/3 and WASP complex at F-actin branches. Cortactin promotes cell motility and invasion, playing a critical role in invadopodia formation—actin-rich protrusions involved in ECM degradation by cancer cells. It is phosphorylated by Src family kinases at Y421, Y466, and Y482, and by Erk family kinases at S405 and S418.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.